Optimum chance phylogenetic relationships of control area haplotypes produced from market purchased eyeball examples and sequences acquired from understood people.
Phylogenetic relationships had been identical in Bayesian inference, maximum likelihood and maximum parsimony estimates (?lnL = 1399.28612; parsimony size = 141). Support values mentioned above nodes are Bayesian probabilities according to 5,000,000 resamples, maximum chance bootstrap help values centered on 1,000 bootstrap replicates, and maximum parsimony bootstrap help values according to 2,000 bootstrap replicates. Help values are shown just for clades of Sotalia guianensis and Sotalia fluviatilis haplotypes, and also for the monophyly of Sotalia. Branch lengths are proportional to maximum chance sequence divergence; scale shows portion of series divergence. Haplotypes 1–14, highlighted in bold, had been present in our research and correspond to GenBank accession #EU022531–EU022544.
To advance confirm the relationships associated with the unknown sequences, we performed a nonphylogenetic cladistic analysis. We constructed a matrix of autapomorphies for Sotalia fluviatilis, Sotalia guianensis, Inia g. Geoffrensis, Inia g. Humboltiana, and Inia g. Boliviensis predicated on available series information in Genbank and our reference individuals, and performed a populace aggregation analysis ( Davis and Nixon 1992 ).
All eyeball?derived sequences decided by BLAST to be of cetacean origin provided species?specific character that is autapomorphic indicative of molecular positional homologies with Sotalia guianensis with no other types, unambiguously assigning all eyeballs of cetacean origin to Sotalia guianensis indian sex cams. The control area and Cytochrome b information sets have two and six diagnostic internet web sites, correspondingly, that assign all eyeball that is cetacean to Sotalia guianensis and unambiguously distinguish it from Sotalia fluviatilis and Inia spp. ( dining dining Table 1; online Appendix S1, S2).
| Control area | BEL | MAO | PVH | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Variable position | 39 | 136 | 139 | 160 | 175 | 231 | 256 | 269 | 270 | 362 | 381 | 387 | 404 | 463 | 504 | 559 | Haplotype occurence | ||
| Inia spp. | A | T | the | G | C | G | G/A | A | A | T | C | C/T | C/T | C | C/T | A | ? | ? | ? |
| Sotalia fluviatilis | T | T | A | G | C/T | G | C/T | T | C | C/T | C | T | T | C | T | A | ? | ? | ? |
| Sotalia guianensis | C | G/T | A/C | A | C/T | G/A | C/T | C/T | C/T | C/T | C/G | C/T | C/T | C/T | C/T | C/A | 22 | 11 | 1 |
| CR_Hap01 | C | T | A | A | T | A | C | C | C | C | C | T | C | C | T | A | 1 | ? | ? |
| CR_Hap02 | C | T | A | A | T | A | C | T | C | C | C | C | T | C | T | A | 2 | ? | ? |
| CR_Hap03 | C | T | A | A | T | A | C | C | C | C | C | C | T | C | T | the | 6 | 2 | ? |
| CR_Hap04 | C | T | A | A | T | A | C | T | C | C | C | T | C | C | T | A | 3 | 5 | 1 |
| CR_Hap05 | C | T | A | A | T | A | C | T | C | T | C | T | C | C | T | the | 1 | 2 | ? |
| CR_Hap06 | C | T | the | A | C | the | T | T | T | C | C | T | T | C | T | the | 1 | ? | ? |
| CR_Hap07 | C | T | A | A | T | A | C | T | C | C | C | C | C | C | T | A | 3 | ? | ? |
| CR_Hap08 | C | G | C | A | T | G | C | T | C | C | C | T | C | C | T | the | 1 | ? | ? |
| CR_Hap09 | C | T | A | A | T | G | C | T | C | C | C | T | C | C | T | the | 1 | ? | ? |
| CR_Hap10 | C | T | A | A | T | A | C | C | C | C | C | T | T | C | T | the | 1 | ? | ? |
| CR_Hap11 | C | T | A | A | T | A | C | C | C | C | C | C | T | T | T | A | ? | 1 | ? |
| CR_Hap12 | C | T | A | A | T | A | C | C | C | C | C | C | T | C | T | C | ? | 1 | ? |
| CR_Hap13 | C | T | A | A | C | the | C | T | T | C | C | T | T | C | C | A | 1 | ? | ? |
| CR_Hap14 | C | T | A | A | T | A | C | C | C | C | G | C | T | C | T | A | 1 | ? | ? |
| Cytochrome b | |||||||||||||||||||
| adjustable position | 49 | 78 | 124 | 297 | 367 | 396 | 462 | 501 | 528 | 531 | 561 | ||||||||
| Inia spp. | G | T | A | C | G | C | T | T | A | C | T | ? | ? | ? | |||||
| Sotalia fluviatilis | G | C | A | G | G | C | T | the | A | C | T | ? | ? | ? | |||||
| Sotalia guianensis | G/A | C/T | G/A | A | G/A | C/T | C | G | C | T | C | 16 | 7 | 1 | |||||
| CB_Hap01 | G | C | A | A | G | C | C | G | C | T | C | 8 | 4 | 1 | |||||
| CB_Hap02 | A | C | A | the | G | C | C | G | C | T | C | 1 | ? | ? | |||||
| CB_Hap03 | G | T | A | A | G | C | C | G | C | T | C | 1 | ? | ? | |||||
| CB_Hap04 | G | C | G | A | G | T | C | G | C | T | C | 3 | 1 | ? | |||||
| CB_Hap05 | G | C | G | A | A | T | C | G | C | T | C | 3 | 2 | ? | |||||
- Internet web web Sites highlighted in bold unambiguously assign the eyeball that is unknown to Sotalia guianensis. Those perhaps maybe not highlighted differentiate haplotypes that are observed. Control area haplotypes 1–14 match GenBank accession numbers EU022531?EU022544, while cytochrome b haplotypes 1–5 match GenBank accession numbers EU022545?EU022549. BEL = Belem, MAO = Manaus, PVH = Porto Velho.
